Haptoglobin polymorphisms and their relationship to the activities of liver function enzymes in sickle cell anemia and hepatitis C patients

СтатьяВОПР ГЕМАТ ОНКО ИММУНОПАТ ПЕДИАТР. 2024; Т. 23, № 4: 72–77. DOI:10.24287/1726-1708-2024-23-4-72-77

Jumaa S.S.^[1]

Maktoof A.A.^[2]

Nuhiar R.S.^[2]

University of Thi Qar College of Vet Medicine

^[1]University of Thi Qar Collage of Vet Medicine

^[2]Department of Biology College of Science University of Thi Qar

The importance of the research lies in the fact that it aims to study the genetic polymorphisms of the haptoglobin (Hp) gene in patients with sickle cell anemia, hepatitis C, and sickle cell anemia with hepatitis C, as well as to study the relationship between the polymorphisms of the gene and the liver enzymes (alkaline phosphatase, aspartate aminotransferase, and alanine transaminase). Hp is a type of alpha-2 globulin found in human plasma. Its primary function is to bind to the globin portion of free hemoglobin in the bloodstream. Objectives: Determining the genotypes of the Hp gene in patients with sickle cell anemia and hepatitis C using allele-specific polymerase chain reaction and studying the relationship between genetic polymorphisms and increased liver enzymes (alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase). This study included 130 participants. They were classified to 4 groups: patients with sickle cell anemia (n = 40), patients with hepatitis C (n = 40), sickle cell patients with hepatitis C (n = 10), and a control group (n = 40). DNA was isolated and polymerase chain reaction was performed using genotype-specific primers for the three regions of the Hp gene. The genotypes were determined after electrophoresis on agarose gel and determination of the amplified fraction of each allele. Alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase levels were measured by colorimetric methods. The results showed that the Hp2-2 genotype was more frequent in all three patient groups than the Hp1-1 or Hp2-1 genotypes. The liver enzyme levels were also significantly higher in the Hp2-2 genotype group than in the other two groups. Hp2-2 was the most prevalent Hp phenotype among the patient groups and it may play a role in the pathogenesis of sickle cell anemia and hepatitis C. The study was approved by the Research Committee of the Thi-Qar Institutional Health Department in 2022.

polymorphism

haptoglobin gene

sickle cell diseases

hepatitis C

Olatunya O.S., Albuquerque D.M., Santos M.N., Kayode T.S., Adekile A., Costa F.F. Haptoglobin gene polymorphism in patients with sickle cell anemia: findings from a Nigerian cohort study. Appl Clin Genet 2020; 8: 107–14.

Kengne Fotsing C.B., Pieme C.A., Biapa Nya P.C., Chedjou J.P., Dabou S., Nguemeni C., et al. Relation between haptoglobin polymorphism and oxidative stress status, lipid profile, and cardiovascular risk in sickle cell anemia patients. Health Sci Rep 2022; 5 (1): e465.

Al-Moussawi D.K. Correlation of HCV Infection and Creatinine Levels in Thalassemia Patients. Thi-Qar J Sci 2022; 9 (2): 80–3.

Sadeghi A., Taherifard E., Dehdari Ebrahimi N., Rafiei E., Hadianfard F., Taherifard E. Effects of L‐arginine supplementation in patients with sickle cell disease: A systematic review and meta‐analysis of clinical trials. Health Sci Rep 2023; 6 (4): e1167.

Al-Badry B.J. Prevalence of antiHBV antibodies in multi-transfused patients with thalassemia at Thi-Qar province. Thi-Qar J Sci 2014; 4 (3): 14–7.

Willen S.M., McNeil J.B., Rodeghier M., Kerchberger V.E., Shaver C.M., Bastarache J.A., et al. Haptoglobin genotype predicts severe acute vaso-occlusive pain episodes in children with sickle cell anemia. Am J Hematol 2020; 95 (4): E92.

Zhou J., Liu J., Sheng H., You N., Chen J., Mi X., et al.; Chinese NAFLD Clinical Research Network (CNAFLD CRN). Haptoglobin 2-2 Genotype is Associated with More Advanced Disease in Subjects with Non-Alcoholic Steatohepatitis: A Retrospective Study. Adv Ther 2019; 36 (4): 880– 95.

Wang Y., Kinzie E., Berger F.G., Lim S.K., Baumann H. Haptoglobin, an inflammation-inducible plasma protein. Redox Rep 2001; 6 (6): 379– 85.

Kingsley D.A., Ofem E., Bassey O.B., Oluwakorede B., Riman O. Biochemical Assessment of the Liver in SCD in a Tertiary Hospital in South-South, Nigeria. J Adv Med Res 2019; 29 (7): 48624.

Jain S.K., Pemberton P.W., Smith A., McMahon R.F.T., Burrows P.C., Aboutwerat A., Warnes T.W. Oxidative stress in chronic hepatitis C: not just a feature of late stage disease. J Hepatol 2002; 36 (6): 805–11.

Louagie H.K., Brouwer J.T., Delanghe J.R., De Buyzere M.L., Leroux-Roels G.G. Haptoglobin polymorphism and chronic hepatitis C. J Hepatol 1996; 25 (1):10–4.

Adekile A.D., Haider M.Z. Haptoglobin gene polymorphisms in sickle cell disease patients with different bS-globin gene haplotypes. Med Princ Pract 2010; 19 (6): 447–50.

Van Vlierberghe H., Delanghe J.R., De Bie S., Praet M., De Paepe A., Messiaen L., et al. Association between Cys282Tyr missense mutation and haptoglobin phenotype polymorphism in patients with chronic hepatitis C. Eur J Gastroenterol Hepatol 2001; 13 (9): 1077–81.

Kamble C.G., Ivvala A.S., Gamit D., Malapati B. Serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase variations with their Pearson’s coefficient correlation in sickle cell disease. J Med Sci Res 2022; 10 (1): 25–9. 16. Kasvosve I., Speeckaert M.M., Speeckaert R., Masukume G., Delanghe J.R. Haptoglobin polymorphism and infection. Adv Clin Chem 2010; 50: 23–46.

Kumar A., Siddiqi N.J., Alrashood S.T., Khan H.A., Dubey A., Sharma B. Protective effect of eugenol on hepatic inflammation and oxidative stress induced by cadmium in male rats. Biomed Pharmacother 2021; 139: 111588.

Ruiz M.A., Shah B.N., Ren G., Hussain F., Njoku F., Machado R.F., et al. Haptoglobin 1 allele predicts higher serum haptoglobin concentration and lower multiorgan failure risk in sickle cell disease. Blood Adv 2022; 6 (24): 6242–8.

Saadon A.A. Prevalence of viral hepatitis B and C among selected group in Thi-Qar. Thi-Qar J Med2012; 6 (1): 79–89.

Öksüz Z., Üçbilek E., Sami Serin M., Yaraş S., Örekici Temel G., Sezgin O. hsa-miR-17-5p: A Possible Predictor of Ombitasvir/Paritaprevir/ Ritonavir plus Dasabuvir +/–- Ribavirin Therapy Efficacy in Hepatitis C Infection. Curr Microbiol 2022; 79: 186.

Nakagawa T., Muramoto Y., Hori M., Mihara S., Marubayashi T., Nakagawa K. A preliminary investigation of the association between haptoglobin polymorphism, serum ferritin concentration and fatty liver disease. Clinica Chimica Acta 2008; 398 (1–2): 34–8.

Philippe M.A., Ruddell R.G., Ramm G.A. Role of iron in hepatic fibrosis: one piece in the puzzle. World J Gastroenterol 2007; 13 (35): 4746.

Johnkennedy N., Odera N.C., Muodebe N.C. Pattern of hepatic enzymes profile in sickle cell disease patients attending Madonna University Teaching Hospital (MUTH). Asian J Res Biol 2022; 5 (1): 34–8.

Louagie H.K., Brouwer J.T., Delanghe J.R., De Buyzere M.L., Leroux-Roels G.G. Haptoglobin polymorphism and chronic hepatitis C. J Hepatol 1996; 25 (1): 10–4.

Ooi K., Shiraki K., Morishita Y., Nobori T. High‐molecular intestinal alkaline phosphatase in chronic liver diseases. J Clin Lab Anal 2007; 21 (3): 133–9.

Reda F.M., El-Saadony M.T., El-Rayes T.K., Attia A.I., El-Sayed S.A., Ahmed S.Y., et al. Use of biological nano zinc as a feed additive in quail nutrition: biosynthesis, antimicrobial activity and its effect on growth, feed utilization, blood metabolites and intestinal microbiota. Italian J Animal Sci 2021; 20 (1): 324–35.

Mohamed A.A., Omar A.A., El-Awady R.R., Hassan S.M., Eitah W.M., Ahmed R., et al. MiR-155 and MiR-665 role as potential non-invasive biomarkers for hepatocellular carcinoma in Egyptian patients with chronic hepatitis C virus infection. J Transl Int Med 2020; 8 (1): 32–40.

Meher S., Mohanty P.K., Patel S., Das K., Sahoo S., Dehury S., et al. Haptoglobin genotypes associated with vaso-occlusive crisis in sickle cell anemia patients of Eastern India. Hemoglobin 2021; 45 (6): 358–64.

Obi C., Aladeyelu O., Agbiogwu I., Agu C.N., Arusiwon J.A., Udeh M.O. Enzyme activities of liver function (Bio‐makers) in sickle cell anaemic patients attending Sickle Cell Anaemic Centre, Benin City, Edo State, Nigeria. Int J Blood Res Disord 2020; 7 (2): 1–5.

Cox S.E., Makani J., Soka D., L'Esperence V.S., Kija E., Dominguez‐ Salas P., et al. Haptoglobin, alpha‐ thalassaemia and glucose‐6‐phosphate dehydrogenase polymorphisms and risk of abnormal transcranial Doppler among patients with sickle cell anaemia in Tanzania. Br J Haematol 2014; 165 (5): 699–706.

Wan B.N., Zhou S.G., Wang M., Zhang X., Ji G. Progress on haptoglobin and metabolic diseases. World J Diabetes 2021; 12 (3): 206–14.

Язык текста: Русский

ISSN: 1726-1708